Priority Research Paper Volume 15, Issue 13 pp 5966—5989

Figure 2. OSK-mediated partial reprogramming ameliorates features of cellular senescence. (A) Heatmaps for mRNA levels of genes upregulated by senescence (n=3, p-adj < 0.01, FC > 2). (B) Percentage of genes changed by OSK (n=3, p-adj < 0.05) among those upregulated by senescence. (C) Heatmaps for mRNA levels of genes downregulated by senescence (p-adj < 0.01, FC > 2). (D) The percentage of genes changed by OSK (p-adj < 0.05) among those downregulated by senescence. (E) Top 20 gene ontology (GO) processes of genes upregulated by senescence. The red and blue bars indicate upregulation or downregulation by OSK, respectively. (F) Top 20 GO processes of genes downregulated by senescence. Red and blue bars indicate upregulation or downregulation by OSK, respectively. (G) Schematics of the Tet-On OSK system integrated in NCC reporter system fibroblasts. (H) NCC signals and track of the arrows in quiescent, senescent, or senescent + OSK fibroblasts. Scale bar, 50 μm. (I) Fluorescence intensity profiles corresponding to the arrow in (H). (J) EGFP intensities in the cytoplasm. Data are mean ± SD. ^*p < 0.05; ^***p < 0.001. One-way ANOVA-Bonferroni.