Research Paper Volume 15, Issue 14 pp 7124—7145

Figure 1. Chronic periodontitis caused the whole body and local Th17/Treg imbalance, and was associated with downregulated lncRNA SPIRE1 expression in mandibular bone marrow–derived mesenchymal stem cells (BM-MSCs). (A, B) The levels of soluble IL-17 (A) and IL-10 (B) in peripheral blood collected from healthy normal mice or periodontitis mice were measured by ELISA. (C, D) The expression levels of RORC mRNA (C) and FoxP3 mRNA (D) in peripheral blood from normal healthy mice or mice with periodontitis were measured by RT-qPCR. (E) Mandibular-BM-MSCs (but not blood cells) from periodontitis displayed a lower expression level of LncRNA SPIRE1 than that from normal control. The expression level of LncRNA SPIRE1 in mandibular BM-MSCs and peripheral blood of the indicated groups were quantitated by qPCR. PBMCs, peripheral blood mononuclear cells. n = 10 for normal healthy mice; n = 10 for periodontitis mice; ^***P < 0.001, between the indicated groups.