Research Paper Volume 15, Issue 14 pp 7084—7097

Figure 3. Recovered mice showed a low anticancer response in the lung. (A) Representative immunohistochemistry images of CEA-immunoreactive cells in lung sections of different groups. Scale bars, top, 100 μm; bottom, 20 μm. (B) Carcinoembryonic antigen (CEA) content in lung tissue homogenate. (C) Schematic of the experimental timeline. The tumor cells were then injected intravenously into untreated and 30D post-LPS treated mice to establish lung metastatic tumor model. (D) In vivo bioluminescence imaging of the B16F10-Luc lung metastasis tumor model in untreated and recovered groups and (E) corresponding quantification results. (F) Representative lung photographs, (G) number of lung tumor lesions, and (H) H&E-stained lung slices. Scale bars: 250 μm. Data are shown as the mean ± SD (n=4-6). Statistical significance was calculated by Student’s t test (two-tailed) and one-way ANOVA using the Tukey posttest. **P< 0.01; ****P < 0.0001.