Research Paper Volume 15, Issue 14 pp 7084—7097
Severe pneumonia induces immunosenescence of T cells in the lung of mice
Figure 2. Senescence of the lungs after severe pneumonia. (A) Western blot analysis of the expression of various types of proteins in lung tissues after various treatments as indicated and the relative expression of proteins compared to the untreated group (n=3). (B, C) p21 and p16 mRNA expression analysis by qPCR (n=3). (D) Flow cytometric analysis for MCP-1+ in CD3+ cells of lung tissue and (E) corresponding quantification results (n=4). Data are shown as the mean ± SD. Statistical significance was calculated by Student’s t test (two-tailed) and one-way ANOVA using the Tukey posttest. *P < 0.05; **P< 0.01.