Research Paper Volume 15, Issue 14 pp 6921—6932

Figure 5. Exosomal lnc-CDHR of hUMSCs alleviates HG-induced EMT by down-regulating miR-3149 and up-regulating PTEN. (A) Transfection efficiency of exosomal lnc-CDHR extracted from transfected stem cells was detected by PCR. (B, C) The effect of MSC CDHR siRNA exosomes on the expression of EMT and PTEN in HG-induced HMrSV5 was detected by WB and PCR. (D, E) Lnc-CDHR and miR-3149 expression were detected by PCR after treatment of HG-induced HMrSV5 with MSC CDHR siRNA exosomes. (F) Transfection efficiency of pc-DNA CDHR was detected by PCR. (G–J) Expressions of Vimentin, α-SMA, E-cadherin, PTEN, CDHR, and miR-3149 were detected by WB and PCR after transfection of pc-DNA CDHR in HG-induced HMrSV5. (K) Transfection efficiency of miR-3149 inhibitor was detected by PCR. (L–O) To illustrate the effect of miR-3149 on EMT, changes of EMT, PTEN, CDHR, and miR-3149 were detected by WB and PCR after miR-3149 inhibition. Each value represents the mean ± SEM (n=3) (^**P<0.01 vs. Control, ^##P<0.01 vs. HG, ^ns no significance vs. HG, (C–E) ^%%P<0.01 vs. HG+NC exo, (H–J) ^%%P<0.01 vs. HG+NC CDHR, (M–O) ^%%P<0.01 vs. HG+NC miR3149).