Figure 3. Single-cell analysis reveals the expression of differential genes in different cell types. (A) Using the tSNE algorithm to dimensionalize the samples into 12 clusters. (B) Heat map clearly showing the major differentially expressed genes in different clusters. (C) The Seurat package annotates different clusters with a total of 4 classes of cells. (D) The bubble diagram shows the expression of difference genes in different cells. (E) Visualization of 13 differential genes by single-cell sequencing.