Research Paper Volume 15, Issue 14 pp 6798—6821

Figure 9. Validation of DNA sensors in cellular DNA damage models was performed by RT-qPCR. (A) DNA damage was verified by comet assay. (B) Relative mRNA expression of IFI16. (C) PRKDC. (D) DHX9. (E) DDX41. (F) cGAS. (G) HNRNPA2B1. (H) DHX36. (I) DDX60. The experimental data were represented by mean ± SD from at least three independent experiments, and the asterisks represent the P-value (**p < 0.01, ***p < 0.001, **** p < 0.0001). The statistical analyses were performed by the student t-test. RT-qPCR, real-time quantitative polymerase chain reaction; IFI16, interferon gamma inducible protein 16; PRKDC, protein kinase, DNA-activated, catalytic subunit; DHX9, DExH-box helicase 9; DDX41, dead-box helicase 41; cGAS, cyclic GMP-AMP synthase; HNRNPA2B1, heterogeneous nuclear ribonucleoprotein A2/B1; DHX36, deah-box helicase 36; DDX60, DExD/H-Box helicase 60.