Research Paper Volume 15, Issue 13 pp 6545—6576
Comprehensive prognostic and immunological analysis of Ubiquitin Specific Peptidase 28 in pan-cancers and identification of its role in hepatocellular carcinoma cell lines
Figure 10. Interfering with the expression of USP28 inhibited cell lines proliferation, migration, and invasion. (A) The protein level of USP28 in HCC and normal tissues. (B) Relative mRNA expression of USP28 in HCC tissues compared to normal tissues. (C) Immunohistochemical staining of USP28 in HCC tissue and adjacent tissue. (D) qPCR and Western blotting analysis of USP28 mRNA and protein expression in four HCC cell lines (HCCLM3, Li-7, Huh-7, Hep3B) and normal liver cell line (HL7702). GAPDH was used as an internal control. (E) The efficiency of USP28 siRNA (si-USP28) in HCCLM3 and Hep3B was confirmed by Western blotting. (F) EdU assays for HCCLM3 and Huh-7 were performed to evaluate cell proliferation ability after transfecting si-USP28. (G, H) Scratch wound healing assay and transwell assays assessed the migration and invasion abilities in HCCLM3 and Huh-7 cells. (Original magnification, ×200; scale bars, 50 μm). *p < 0.05; ** p < 0.01; *** p < 0.001.