Research Paper Volume 15, Issue 13 pp 6400—6428
Exploring Cancer Dependency Map genes and immune subtypes in colon cancer, in which TIGD1 contributes to colon cancer progression
Figure 10. Gene effect scores based on CRISPR-Cas9 and Silencing TIGD1 gene inhibited CC cells proliferation and cycle. (A) The interference values of five CDM risk genes. (B) The interference values of colon cancer cell lines (DLD1, HCT116, HT29, SW620). (C) siRNAs interfered TIGD1 expression in HCT116 and SW620 (three group: nc, si988 and si1170). (D, E) HCT116 and SW620 cells were significantly inhibited in growth with knocking down the TIGD1 gene expression by cck8 assays. (D) for HCT116, (E) for SW620. (F) The cloning ability of HCT116 and SW620 cell lines were significantly inhibited with knocking down the TIGD1 gene expression by clone formation assays. (G) Silencing the expression of TIGD1 gene retarded the proliferation cycle of HCT116 and SW620 in the G1 phase.