Figure 2.DSC2 suppressed viability of GC cells in vitro. After stably expressing DSC2 gene, the viability of cells was determined by colony formation assay (A, in MGC-803 cells), MTT assay, Caspase-3 activity assay and sperm DNA fragmentation assay (B–D, in MGC-803 and SGC-7901 cells). (E, F) The expression of BCL-2 and Cyto-C were determined by Western blot assay. Data are presented as mean ± SEM from three separate experiments. **p<0.01 and ***p<0.001 vs. BC. ##p<0.01 and ###p<0.001 vs. Lenti-NC group. BC: blank control group, Lenti-NC: lentivirus negative control group.
