Research Paper Volume 15, Issue 13 pp 6331—6345
MIR34A modulates lens epithelial cell apoptosis and cataract development via the HK1/caspase 3 signaling pathway
Figure 4. Mir34a modulates the opacification of the lens via the regulation of Hk1 expression. (A) The lens explant of the mouse was co-cultured with Mir34a, siHk1, or deoxyglucose, then the opacification of the lens was observed. (B) Lens explants of the mouse were performed as A, and Hk1 expression on lens epithelium was detected by immunofluorescence. Scale bars: 20 μm. (C) Lens explants of the mouse were performed as A, and the apoptosis of lens epithelium was detected by TUNEL. Scale bars: 20 μm. ***P < 0.001.