Research Paper Volume 15, Issue 13 pp 6302—6330

Figure 8. ADAR was up-regulated in bladder cancer tissues and served as a prognostic factor in bladder cancer. (A) Relative expression of ADAR mRNA in the 40 pairs of bladder cancer tissues and matched adjacent normal tissues quantified by qRT-PCR. ADAR was up-regulated in bladder cancer tissues compared with that in adjacent normal tissues. (B) The expression of ADAR protein in 8 pair bladder cancer tissues (T) and adjacent normal tissues (N) by western blot were shown. (C) Relative expression of ADAR in bladder cancer cell lines and normal bladder epithelial cell line SV-HUC-1 by qRT-PCR. Data represent the mean ± SD from three independent experiments, ^*P < 0.05. (D) IHC analysis of ADAR in bladder cancer tissue at 100× and 400× magnification. (E) Kaplan-Meier survival curves of overall survival in 180 bladder cancer patients based on ADAR by IHC staining. The log-rank test was used to compare differences between two groups (P = 0.0028). (F, G) Validation of the knockdown efficacy of ADAR in T24 and BIU87 cell lines by qRT-PCR and western blot. Data represent the mean ± SD from three independent experiments, ^*P < 0.05. (H, I) The overexpression efficacy of ADAR in T24 and BIU87 cell lines by qRT-PCR and western blot. Data represent the mean ± SD from three independent experiments, ^*P < 0.05.