Research Paper Volume 15, Issue 13 pp 6225—6254

Figure 8. COL1A2 promotes the proliferation, migration, and invasion of GBM cells in vitro. (A–C) Differential expression of COL1A2 at transcriptional (A) and protein levels (B, C) between different GBM cell lines. (D–F) The efficiency of COL1A2 knock-down plasmid at transcriptional (D) and protein levels (E, F) was verified in U87 cells. (G–I) The efficiency of the COL1A2 overexpression plasmid at transcriptional (G) and protein levels (H, I) was verified in U251 cells. In (A–I), data are presented as the mean ± SD. X-axis: four different cell lines. Y-axis: expression level of genes. (J–Q) The proliferation activity of U251 and U87 cells were detected by colony formation assay (J–M), CCK-8 assay (N, O), and EdU staining (P, Q). (R–U) The migration and invasion ability of U87 and U251 cells were detected by wound healing (R, S) and Transwell assays (T, U). The representative photographs were photographed under a microscope. *P<0.05, **P<0.01, ***P<0.001.