Research Paper Volume 15, Issue 13 pp 6031—6072

Figure 5. Enrichment improves hippocampus-dependent spatial working memory via MSK1. (A) In the Adult groups the percentage of correct alternations was higher for mice in enrichment (Housing: F(1,61) = 6.10 p = 0.016). Although there was no significant interaction, the difference between EE and SH was greater in the WT mice, which showed a significant improvement (F(1,61) = 5.64 p = 0.021) compared to the MSK1 KD which showed no significant (ns) improvement in performance compared to their standard-housed counterparts (F(1,61) = 1.27 p = 0.270). (B) In the Aged groups there was a significant interaction of Genotype x Housing F(1,68) = 8.17 p = 0.006. The Simple Main Effect analysis showed that the difference between EE and SH was greater in the WT which showed a highly significant improvement F(1,68) = 15.04 p = 0.0002 compared to the KD which showed none F(1,68) = 0.01 p = 0.920. Results are expressed as arm entries sequence over 10 minutes for which a correct alternation % was calculated with the criterion of one repetition across 5 entries. These results indicate that the benefit of enrichment was exclusive to WT. Individual data points are presented for each animal, with the bar graph representing the mean ± SEM of the data.