Research Paper Volume 15, Issue 13 pp 6011—6030

Figure 1. BUB1 depletion stabilizes EGFR. (A) A549 cells were transfected with non-targeting control scrambled (NSS) siRNA or BUB1siRNA. 48 hours post-transfection cells were cross-linked with 100 μM DSS for 30 minutes followed by 30 ng/mL EGF for an additional 30 minutes. Resulting lysates were resolved on SDS-PAGE gels and probed with indicated antibodies. (B) Quantitation of western blots from (A) only the monomer species of EGFR is plotted. Control siRNA (NSS) transfected, EGF treated lanes were set as 1 fold and used as a baseline for estimating fold enrichment in other samples. (C) Quantitation of pEGFR and EGFR dimers from SDS and EGF treated lanes only (lanes 4 and 8 only in A). NSS transfected lane was set as 1 fold and used as a baseline for estimating fold enrichment in BUB1 siRNA transfected samples. (D) Gene expression values from non-metastatic adenocarcinoma samples (N=331) from TCGA lung dataset were log₂ transformed and median centered and correlation coefficient (r) was calculated. BUB1 and EGFR expression is expressed as log₂ transformed values. Correlation coefficient and p-value are listed.