Research Paper Volume 15, Issue 12 pp 5611—5649
Identifying the role of NUDCD1 in human tumors from clinical and molecular mechanisms: a study based on comprehensive bioinformatics and experimental validation
Figure 10. Validation of NUDCD1 expression and function in STAD. (A) Relative NUDCD1 expression in STAD tissues and adjacent pericarcinous tissues. (B) Immunohistochemistry analysis of NUDCD1 expression in STAD tissues. (C) Relative NUDCD1 expression in the normal gastric mucosal cell line, GES-1, and four STAD cell lines, AGS, HGC-27, MKN28 and SGC7901. (D) Relative mRNA and (E) protein expression of NUDCD1 in AGS and HGC-27 cells with LV3-NC or LV3-sh-NUDCD1. (F) The ratio of apoptosis in AGS and HGC-27 cells with LV3-NC or LV3-sh-NUDCD1. (G) Cell cycle in AGS and HGC-27 cells with LV3-NC or LV3-sh-NUDCD1. (H) Colony formation assay results in AGS and HGC-27 cells with LV3-NC or LV3-sh-NUDCD1. (I) Weight of subcutaneously xenografted STAD in nude mice.