Research Paper Volume 15, Issue 12 pp 5535—5549
Galectin-1-mediated high NCAPG expression correlates with poor prognosis in gastric cancer
Figure 4. LGALS1 and NCAPG may mutually reinforce regulation in GC cell lines. qRT-PCR analysis of (A, B) NCAPG and (C, D) LGALS1 expression in SGC-7901 and HGC-27 cells when LGALS1 was overexpressed, with or without simultaneous silencing of NCAPG. Western blot confirmation of stable overexpression of Gal1 and NCAPG in (E) SGC-7901 and (F) HGC-27 cells when LGALS1 was overexpressed, with or without simultaneous silencing of NCAPG. Abbreviations: OE-LGALS1: Overexpression of LGALS1; siNCAPG: silencing of NCAPG; OE-LGALS1+NC: overexpression of LGALS1 + NCAPG negative control (empty vector); NS: not significant. *P < 0.05; **P < 0.01.