Research Paper Volume 15, Issue 12 pp 5426—5444

Figure 6. XMU-MP-1 reverses inhibition and apoptosis of TE-1 and KYSE150 cells proliferation by chaetocin. (A) TE-1 and KYSE150 cells were treated with chaetocin (0.8 μM) in the presence and absence of 1 μM XMU-MP1. The cell viability was examined by CCK-8. The colony formation was evaluated after 7 days. (B) Apoptosis was measured by flow cytometry. Results were shown as mean ± SD of three independent experiments. ^**P < 0.01, ^***P < 0.001. (C) Expression levels of Hippo pathway proteins were analyzed through western blot. GAPDH was used as the loading control. (D) Expression levels of PARP, Cleaved PARP were detected by western blot. GAPDH was used as the loading control.