Research Paper Volume 15, Issue 12 pp 5426—5444

Figure 5. The Hippo pathway is involved in chaetocin-induced ESCC cells apoptosis and anti-proliferation. (A) RNA-seq was performed on non-treated TE-1 cells as well as TE-1 cells with 0.4 μM and 0.8 μM chaetocin treatment for 24 h. Volcano plots were used to analyze transcriptomic data, with the x-axis representing Log2FoldChange (sample/control) values and the y-axis representing the -Log10 (padj). Green, red, and gray circles respectively represent genes that were downregulated, upregulated, and not differentially regulated. (B) Venn diagrams demonstrating the numbers of up- and down-regulated transcripts associated with each treatment. (C) KEGG pathway enrichment analysis of DEGs that were specifically downregulated in the chaetocin treatment. (D) Representative western blot results showing changes in phosphorylation level of proteins in the Hippo pathway, including MST1/2, MOB1, LATS1, SAV1, and YAP, after chaetocin treatment. GAPDH was used as the loading control. (E) Cytosolic and nuclear proteins of TE-1 and KYSE150 cells treated with 0.8 μM chaetocin for 24 h were separated to detect expression levels of YAP. GAPDH and PCNA were used as the loading controls.