Research Paper Volume 15, Issue 12 pp 5412—5425
Tumor-suppressive function and mechanism of miR-873-5p in glioblastoma: evidence based on bioinformatics analysis and experimental validation
Figure 3. HMOX1 up-regulates SPOP expression by promoting HIF1α expression. (A) Expression of HIF1α in normal brain tissue samples and GBM tissue samples analyzed by the GEPIA database. The red box represents the GBM tissue samples (n = 163) and the gray box represents the normal brain tissue samples (n = 207). (B) Transfection efficiency of sh-HMOX1 in T98G cells determined by Western blot. (C) Expression of SPOP in normal brain tissue samples and GBM tissue samples analyzed by the GEPIA database. The red box represents the GBM tissue samples (n = 163), and the gray box represents the normal brain tissue samples (n = 207). (D) Western blot of SPOP protein in tumor tissues of GBM patients (n = 20) and normal brain tissues (n = 20). (E) Western blot of SPOP protein in HEB and T98G cells. (F) Enrichment of SPOP promoter using anti-HIF1α antibody detected by ChIP assay. (G) Transfection efficiency of sh-HIF1α in T98G cells determined by Western blot. (H) Western blot of HMOX1, HIF1α and SPOP proteins in T98G cells transfected with oe-HMOX1 alone or combined with sh-HIF1α. *p < 0.05, compared to normal brain tissues, HEB cells or T98G cells transfected with sh-NC or oe-NC + sh-NC. #p < 0.05, compared to oe-HMOX1 + sh-NC-transfected T98G cells. The cell experiment was run in triplicate independently.