Research Paper Volume 15, Issue 11 pp 5215—5227

Figure 4. Alterations in PUS1 expression affect A498 cell and ACHN cell viability, migration, invasion, colony formation, and the expression of E-Cadherin and Vimentin. (A) The protein expression of PUS1 in different RCC cell lines. (B) The mRNA expression levels of PUS1 in cancer tissues and adjacent normal epithelial tissues from patients with RCC. N = 10. (C) A498 cells are infected with PUS1-LV and PUS1 expression was detected by western blot. (D) ACHN cells are infected with PUS1-shRNA-LV and PUS1 expression was detected by western blot. (E) A498 cells are infected with PUS1-LV and cell viability was measured. (F) ACHN cells are infected with PUS1-shRNA-LV and cell viability was measured. (G) A498 cells are infected with PUS1-LV and cell migration was determined by wound healing assay. (H) Quantitative statistics of (G). (I) A498 cells are infected with PUS1-LV and cell invasion was detected by matrigel-transwell assay. (J) Quantitative statistics of (I). (K) ACHN cells are infected with PUS1-shRNA-LV and cell migration was determined by wound healing assay. (L) Quantitative statistics of (K). (M) ACHN cells are infected with PUS1-shRNA-LV and cell invasion was detected by matrigel-transwell assay. (N) Quantitative statistics of (M). (O) A498 cells are infected with PUS1-LV and cell colony formation was performed. (P) Quantitative statistics of (O). (Q) ACHN cells are infected with PUS1-shRNA-LV and cell colony formation was performed. (R) Quantitative statistics of (Q). (S) Protein levels of E-Cadherin and Vimentin in A498 cells infected with PUS1-LV. (T) Protein levels of E-Cadherin and Vimentin in ACHN cells infected with PUS1-shRNA-LV. SD, error bar; ^**p < 0.01; ^*p < 0.05.