Research Paper Volume 15, Issue 11 pp 4685—4698

Figure 1. Age does not decrease LAMP2A levels in UM-HET3 or C57BL/6J mice. (A) Representative western blots and quantifications of LAMP2A and HSPA8 are shown in whole livers lysates from ad libitum fed male and female UM-HET3 mice of ages 4, 14, and 24 months. H3 and ENO1 are loading controls. n = 6 animals per group. (B) Representative western blots and quantifications of LAMP2A and HSPA8 are shown in whole kidney lysates from ad libitum fed male and female UM-HET3 mice of ages 4, 14, and 24 months. H3 is a loading control. n = 6 animals per group. (C) Representative western blots and quantifications of LAMP2A and HSPA8 are shown in whole brain lysates from ad libitum fed male and female UM-HET3 mice of ages 4, and 24 months. H3 and ACTB are loading controls. n = 6 animals per group. (D) Representative western blots and quantifications of LAMP2A and total LAMP2 are shown in whole livers lysates from ad libitum fed male C57BL/6J mice of ages 2, 8, and 24 months. ACTB is a loading control. n = 9 for 2- and 24-month groups, n = 8 for 8-month group. Statistical analysis was performed in GraphPad Prism 9. Lines are drawn at each mean, with error bars showing S.E.M. p-values derived from 2-way ANOVAs of both “full models” and “main effects models” are shown beneath each graph. “Estimation plots” are shown to the right of each graph; error bars on estimation plots show the 95% C.I. for the difference between the means of the indicated groups. p values displayed directly on the graphs are derived from unpaired t tests.