Research Paper Volume 15, Issue 13 pp 6100—6116

Figure 6. The regulatory mechanism of the lncRNA MCF2L-AS1-miR-33a-5p-FGF2 axis. (A) Western blot detected FGF2 protein expression in liver cancer tissues. (B) Targetscan forecast a binding site between miR-33a-5p and FGF2. (C) qRT-PCR checked the transfection efficiency of miR-33a-5p inhibitors and mimics. (D) The luciferase activity of 293T cells transfected with different vectors was confirmed through dual-luciferase reporter assay. (E) qRT-PCR checked the mRNA level of FGF2. (F, G) Western blot detected FGF2 protein expression. (H) The luciferase activity of 293T cells transfected with MCF2L-AS1-WT, MCF2L-AS1-Mut, the miR-33a-5p inhibitor or negative control was ascertained through dual-luciferase reporter assay. (I) qRT-PCR checked the mRNA level of FGF2. (J, K) Western blot detected FGF2 protein expression. (L, M) Pull-down assay and qRT-PCR detected the regulatory relationship between MCF2L-AS1 and miR-33a-5p. (**, P<0.01, the difference is statistically significant).