Research Paper Volume 15, Issue 13 pp 6100—6116
MCF2L-AS1 promotes the biological behaviors of hepatocellular carcinoma cells by regulating the miR-33a-5p/FGF2 axis
Figure 5. Effects of MCF2L-AS1-miR-33a-5p on MHCC97H cell proliferation, invasion and apoptosis. miR-33a-5p mimics and/or MCF2L-AS1 overexpression plasmids were transfected into MHCC97H cells. (A) miR-33a-5p and MCF2L-AS1 expression levels were verified by qRT-PCR. (B) CCK8 detected MHCC97H and HCCLM3 cell proliferation. (C, D) Colony formation experiment was conducted for detecting cell colony formation ability. (E, F) EdU staining was performed for evaluating cell proliferation. Scale bar=50 μm. (G–J) Transwell assay detected MHCC97H cell migration and invasion, Scale bar=200 μm. * P<0.05, **P<0.01, *** P<0.001. N=3.