Research Paper Volume 15, Issue 11 pp 4667—4684

Figure 2. Expression patterns of selected genes during differentiation of C2C12 myotubes. (A) Relative gene expression of target genes in differentiating C2C12 cells at 0-, 1-, 2-, 3-, and 5-days post-induction. mRNA levels were normalized to 36b4 mRNA levels, as measured by qPCR analysis. The data are represented as mean ± SEM. Statistically significant differences are denoted as ^*p < 0.05, ^**p < 0.01, ^***p < 0.005 vs. DM0. (B) Representative images of myosin heavy chain (MHC; green) immunostaining in myoblasts transfected with target genes and differentiated for 5 days. (C) Quantification of myotube myosin-positive (Myh+) areas, (D) myotube diameter, and (E) fusion index. (F) Western blot showing expression of MHC and myogenin, differentiation markers, during differentiation (0-, 1-, 2-, and 3-days). The data are represented as mean ± SEM. Statistically significant differences are denoted as ^*p < 0.05, ^**p < 0.01, ^***p < 0.005 vs. si-Con.