Research Paper Volume 15, Issue 11 pp 5096—5124

Figure 10. Knockdown of HOXB9 expression inhibits cells proliferation and invasion. (A) HOXB9 expression levels in GBM tissues and adjacent normal tissues. (B, C) Quantification analysis of HOXB9 protein and mRNA expression in eight paired GBM tissues and their adjacent normal tissues. (D) Representative H&E and IHC staining of HOXB9 in GBM and paired normal tissues (magnification ×200). Scale bar, 100 μm. (E) The results of western blotting and qRT-PCR detected the levels of HOXB9 expression in glioma cell lines (SW1783, U87, and SW1088) compared with normal human astrocytes (NHA). (F) The HOXB9 expression of the shHOXB9 group is less than that of the shNC group in U87 cells. (G–I) CCK-8 (G), colony formation (H), and EdU assays (I) were used to detect the proliferation ability in U87 cells transfected with shHOXB9 plasmids. (J) Transwell assays detected the migration and invasion ability in U87 cells transfected with shHOXB9 plasmids. ns > 0.05, ^#P < 0.05, ^##P < 0.01.