Research Paper Volume 15, Issue 11 pp 4949—4962
PZR promotes tumorigenicity of lung cancer cells by regulating cell migration and invasion via modulating oxidative stress and cell adhesion
Figure 7. Altered expressions of PZR affect intracellular ROS levels. Wild type (WT), PZR-knockout (PZR-KO), vector-overexpressing (V-OE), and PZR-overexpressing (PZR-OE) SPC-A1 cells were incubated with 10 μM DCFH-DA for 30 min at 37°C in the dark and then viewed under a fluorescence microscope (A, B) or analyzed using a flow cytometer (C, D). Bar graphs show mean flow cytometric fluorescence intensity (magnification, ×100). All assays were done in triplicates. Data in bar graphs represent mean ± SD (n = 3). ****P < 0.0001. (E) Activities of T-SOD in SPC-A1. *P < 0.05.