Research Paper Volume 15, Issue 11 pp 4949—4962
PZR promotes tumorigenicity of lung cancer cells by regulating cell migration and invasion via modulating oxidative stress and cell adhesion
Figure 2. Generation of PZR-knockout SPC-A1 cells. (A) Two sgRNAs targeting exon 2 of the MPZL1 gene were designed for CRISPR genome editing. (B) Verification of PZR knockout in SPC-A1 cells by Western blotting using antibodies against PZR and β-actin as indicated. (C) PCR analysis of genomic DNA from wild type and PZR-KO SPC-A1 cells primers Pf. and Pr. surrounding the sgRNA-targeting sites. PCR products were analyzed on 2% agarose and visualized with ethidium bromide. (D) DNA sequencing verification of a 43-bp deletion in PZR-KO SPC-A1 cells. Sequencing was performed from the 3′-side with primer Pr. The position of a 43-bp fragment deletion was marked by a red arrow.