Figure 5.Effect of ISO on oxidative stress. (A) Fluorescence imaging analysis and (B) flow cytometry analysis on the ROS production of ISO treatment; (C) Bar graph summarizes the data on ROS production. (D–H) Effects of ISO pretreatment on the Nrf2/keap1 pathway by western blotting analysis. (I, J) Effect of ISO on the level of SOD and the activity of MDA of H2O2-induced injury Chondrocytes viability. (K–N) Effects of ISO pretreatment on the PI3K/Akt pathway by western blotting analysis. Results shown are expressed as means ± SD (n = 3). #p < 0.05 compared with control group, *p < 0.05 compared with OA group. The H2O2 concentration is 500 μM.
