Research Paper Volume 15, Issue 11 pp 4734—4745
miR-506-3p induces autophagy of renal tubular epithelial cells in sepsis through targeting PI3K pathway
Figure 1. Bioinformatics analysis. (A) The gene expression in GSE101639 was subjected to quantile normalization. (B) DEGs in normal group and sepsis group. (C) It was found in GSE154918 that the expression of PIK3CA was down-regulated in sepsis compared with that in normal samples. (D) The upstream miRNAs of PIK3CA were evaluated through miRDB, starBase and TargetScan, and the DEG of miR-506, a key regulator of PIK3CA, was obtained from the intersection. (E) miR-506 of PIK3CA had a targeted binding region. (F) GO enrichment analysis was performed using the online tool DAVID. A total of 37 down-regulated pathways were enriched, including autophagy, transcription elongation from RNA polymerase II promoter and regulation of blood pressure. (G) 30 up-regulated pathways, including protein SUMOylation, glucose homeostasis and negative regulation of protein kinase activity. (H) KEGG pathway analysis was conducted on the integrated DEGs using DAVID. (I) The hub gene was obtained by Cytoscape.