Research Paper Volume 15, Issue 10 pp 4524—4532

Figure 4. Antcin A suppresses liver injury in mice. (A, B) ALT and AST levels (n=10): The ALT and AST levels in ALI mice increased, and the differences were significant compared with Control group. Antcin A treatment suppressed liver injury, and decreased ALT and AST levels, with significant differences between groups (^*P<0.05). (C–E) ELISA (n=10). The levels of inflammatory factors IL-6, IL-1β and TNF-α in ALI mice increased, while Antcin A treatment suppressed the expression of inflammatory factors, with significant differences between groups (^*P<0.05). (F–H) Levels of SOD, MDS and GSH-Px (n=10). Antcin A enhanced the levels of SOD and GSH-Px, but decreased that of MDA, and there were significant difference between groups (^*P<0.05). (I) H&E (n=5). Liver tissues developed inflammation, edema and necrosis in ALI group, with obvious cell injury, while no obvious injury was observed in Control group, and Antcin A reduced inflammatory response and cell injury in tissues. (J, K) Relative protein expression levels (n=5). Antcin A did not significantly affect MAPK1 expression, but it decreased the expression levels of MAPK3. ^*P<0.05 compared with Control group, ^#P<0.05 compared with ALI group.