Research Paper Volume 15, Issue 10 pp 4498—4509
Expression of αA-crystallin (CRYAA) in vivo and in vitro models of age-related cataract and the effect of its silencing on HLEB3 cells
Figure 5. Effect of CRYAA silencing on apoptosis and autophagy. (A, a) Flow cytometry results of apoptosis at 48 h after transfection, sh260 group compared with the normal group, with statistical significance, ** p<0.01; (B, b) CRYAA silencing had no effect on the cell cycle. (C) WB results for apoptosis and autophagy proteins, c and d are the result of the apoptotic proteins Cas3 and Bax, sh260 group compared with the normal group, with statistical significance, ** p<0.01, * p<0.05. e and g are the result of the autophagy proteins Beclin1and P62, f is the result of LC3II/LC3I, sh260 group compared with the normal group, with statistical significance, *** p<0.01, ** p<0.01. There was no difference between ser and con group.