Expression of αA-crystallin (CRYAA) <i>in vivo</i> and <i>in vitro</i> models of age-related cataract and the effect of its silencing on HLEB3 cells

Xiaoling Ma; Yi Nan; Can Huang; Xiangyang Li; Yifan Yang; Wenjie Jiang; Mengyi Ye; Qian Liu; Yang Niu; Ling Yuan

doi:doi:10.18632/aging.204754

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Research Paper Volume 15, Issue 10 pp 4498—4509

Expression of αA-crystallin (CRYAA) in vivo and in vitro models of age-related cataract and the effect of its silencing on HLEB3 cells

Figure 2. CRYAA expression in vivo experiments. (A, B) The expression of CRYAA protein in HLEB3 cells treated with 0, 300, 500, 700μmol/L H₂O₂ for 24 hours and (C, D) 0, 500μmol/L H₂O₂ for 12, 24, 36 hours. (E) The expression of CRYAA mRNA in HLEB3 cells treated with 0, 300, 500, 700μmol/L H₂O₂ for 24 hours; (F) CRYAA mRNA expression in HLEB3 cells treated with 0 and 500μmol/L H₂O₂ for 12, 24, and 36 hours. ns p>0.05, *p<0.05, **p<0.01, ***p<0.001.