Research Paper Volume 15, Issue 12 pp 5355—5380
Construction and experimental validation of a B cell-related gene signature to predict the prognosis and immunotherapeutic sensitivity in bladder cancer
Figure 4. Establishment of the B cell-related gene signature. (A) The workflow showed the process of the predictive model construction. (B) The LASSO regression identified 16 gene-pairs significantly associated with OS in the TCGA-BLCA cohort. (C) The coefficients of the variables in the LASSO regression model. (D) The random forest algorithm identified 38 gene-pairs with VIMP > 0.001. (E) 6 gene-pairs were con-determined by the LASSO and random forest analyses, 5 of which were identified with the multivariate Cox regression with stepwise. (F) The association of the genes in the predictive model with B cells’ infiltration proportion. (G) The expression levels of the genes in the established model in each cell cluster.