Research Paper Volume 15, Issue 10 pp 4411—4428

Figure 6. The impact of miR-194 and E2F3 on DU145 cell proliferation, apoptosis, invasion, and migration. E2F3 overexpression and si-E2F3 plasmids were transfected into DU145 cells for 24 hour culture after miR-194 mimics or inhibitors were administered. (A, B) CCK8 assay checked cell proliferation. (C, D) Transwell assay examined cell migration and invasion. Scale bar = 100 μm. (E, F) TUNEL staining detected cell apoptosis. Scale bar = 50 μm. (G, H) qRT-PCR examined the profiles of lncRNA H19 and miR-194. (I, J) Western blot was used for examining E2F3 protein level. ^***P < 0.001 (vs. NC or NC-in); ^&P < 0.05, ^&&P < 0.01, ^&&&P < 0.001 (vs. miR-149 or miR-149 in), n = 3.