Research Paper Volume 15, Issue 10 pp 4012—4034
Key elements of cellular senescence involve transcriptional repression of mitotic and DNA repair genes through the p53-p16/RB-E2F-DREAM complex
Figure 5. Downregulation of networks of genes associated with chromatin structure. (A) Network generated by IPA shows that multiple histones and genes associated with formation of chromatin structure were downregulated in senescence. (B, C) Western blot analysis of selected genes in serially passaged HUVECs cells (population doubling (PD) PD5-PD30) and TNF-α-mediated senescence (cell treated with various concentration of TNF-α). Actin and tubulin were used as loading controls.