Research Paper Volume 15, Issue 10 pp 4144—4158

Figure 5. Exosomal lncRNA GAS5 from hUC-MSCs regulate suppression on target PTEN genes to alleviate EMT of HPMCs. (A, B) hUC-MSCs were transfected with lncRNA GAS5 siRNA, and the expression of lncRNA GAS5 was detected by real-time PCR in hUC-MSCs (A) and hUC-MSCs exosomes (B). (C) The expression of EMT markers was detected by Western Blot in HPMCs. (D) Statistical results of EMT changes after HG and hUC-MSCs/hUC-MSCs exosomes treatment. (E) The expression of EMT markers was detected by real-time PCR in HPMCs. (F) The expression of lncRNA GAS5 was detected by real-time PCR in HPMCs. (G) The expression of miRNA-21 was detected by real-time PCR in HPMCs. (H) The expression of PTEN was detected by Western Blot in HPMCs. (I) Statistical results of PTEN changes after HG and hUC-MSCs/hUC-MSCs exosomes treatment. Each value represents the mean ± SEM (n = 3) (^**P < 0.01 vs. Control, ^##P < 0.01 vs. HG, ^%%P < 0.01 vs. HG+ NC GAS5i exo, ^%P < 0.05 vs. HG+ NC GAS5i exo).