Research Paper Volume 15, Issue 9 pp 3791—3806

Figure 3. miR-497 suppression is mediated by EZH2 and histone acetylation. (A) Cells were treated with EZH2 inhibitor DZNep, and DZNep treatment greatly increased miR-497 expression levels in the cells in a dose-dependent manner. (B) Compared to vector control, overexpression of EZH2 significantly reduced miR-497 expression. (C) A negative correlation was showed between EZH2 and miR-497 levels in esophageal cancer tissues from the data obtained from TCGA database. (D) The histone deacetylases inhibitor TSA treatment in Eca109 induced miR-497 expression levels. (E) The esophageal cancer data from TCGA was used to analyze correlation between expression levels of miR-497 and histone deacetylases: HDAC1, HDAC2 and HDAC3, which is inversely correlated. Spearman’s correlation analysis was carried out in (C, E). All data were representative of 3 independent experiments. * indicated significant difference at p<0.05, ** indicated significant difference at p<0.01.