Research Paper Volume 15, Issue 9 pp 3331—3355

Figure 1. Zoledronic acid has senolytic effects in human lung fibroblast IMR90 cells. (A) Increasing concentrations (0.04-90 μM) of zoledronic acid were tested for 72 h in IMR90 cells. The figure shows the percentage non-senescent (black) and senescent IMR90 cells (red) remaining after 72 hours of treatment. n = 3; (B) Representative images of C₁₂FDG-based senescence assay of zoledronic acid in senescent IMR90 cells. Blue fluorescence indicates nucleus staining with Hoechst 33324, and bright green fluorescence indicates SA-β-gal positive senescent cells whereas dim green fluorescence represents SA-β-gal low or negative, non-senescent cells. Images were taken using Cytation 1 at 4X.