Research Paper Volume 15, Issue 9 pp 3644—3677

Figure 3. Unsupervised clustering of BMMCs of patient P14. (A) Unsupervised clustering of BMMCs of patient P14 at the single cell transcriptome level. One point means one single cell of P14 BMMCs. C1 and C2 means cluster 1 and cluster 2, showed by two different colors. (B) Silhouette method for cluster selecting. Average silhouette width was calculated for one to ten cluster. The cluster number was chosen with the biggest average silhouette width (dotted line). (C) Lower transcriptome expression level in C1 compared with C2 of single cell transcriptome in P14 patients. P = 0.0003, Mann Whitney test, two sided. (D) FAM46C and immunoglobulin genes were differently expressed between C1 and C2. P < 0.05, Mann Whitney test, two sided.