Research Paper Volume 15, Issue 9 pp 3635—3643
TIMP2 facilitates CIRI through activating NLRP3-mediated pyroptosis
Figure 4. TIMP2 can stimulate NLRP3-mediated cell apoptosis in mice with CIRI. (A) Western blotting is performed to detect the expression levels of TIMP2, p-ERK1/2, NLRP3, and IL-1β in each group. The protein banding diagram of IL-18, GSDMD, Caspase-1, and ASC; (B) The protein expressions of TIMP2, p-ERK1/2, NLRP3, IL-1, IL-18, GSDMD, Caspase-1, and ASC in the control, I/R, I/R+TIMP2, and TIMP2-KD groups. It can be observed that TIMP2 can upregulate the expression of these proteins, indicating that TIMP2 can aggravate cell pyroptosis in mice with CIRI. **P < 0.01.