Research Paper Volume 15, Issue 9 pp 3549—3571

Figure 5. miR-663b targeted AMPK. (A, B) With the wild type (wt) and mutant type (mt) sequences of AMPK mRNA 3′-UTR built, dual luciferase activity assay was implemented to check the luciferase activity of 293T cells transfected along with miR-663b mimics, miR-NC, and wt or mt-AMPK 3′-UTR. (C) Verification of the relationship between miR-663b and AMPK by RNA pull-down assay. (D–H) Western blot confirmed the profile of the AMPK and Sirt1 in PASMCs. N = 3. ns P > 0.05, ^**P < 0.01, ^***P < 0.001 (vs. miR-NC, Blank, or Exo^M0); ⁺P < 0.05, ⁺⁺P < 0.01, ⁺⁺⁺P < 0.001 (vs. miR-NC+hypoxia or miR-in+hypoxia).