Research Paper Volume 15, Issue 9 pp 3465—3479

Figure 3. JDP2 is a direct target of miR-143-5p in ARPE-19 cells. (A) The underlying targets of miR-143-5p predicted using TargetScan, microT, and miRDB online tools followed by Venn analysis. (B) The qPCR assay showed that JDP2 mRNA expression was most significantly decreased in ARPE-19 cells after transfection of miR-143-5p mimics, compared with NC. (C) Diagrams showing the putative binding sites of miR-143-5p and the corresponding wild-type and mutant sites of JDP2 mRNA 3′ untranslated region (UTR). (D) miR-143-5p markedly suppressed the luciferase activity that carried the wild-type (WT) but not the mutant (MUT) 3′ UTR of JDP2. (E) The relative mRNA of JDP2 markedly decreased in ARPE-19 cells after transfection with miR-143-5p mimics (n = 3 for each group). (F) Relative JDP2 protein expression significantly in ARPE-19 cells after transfection with miR-143-5p mimics (n = 3 for each group). ^*P < 0.05, ^**P < 0.01, and ^***P < 0.001, as compared with the indicated NC group.