Research Paper Volume 15, Issue 8 pp 3120—3140
An artificial neural network model to diagnose non-obstructive azoospermia based on RNA-binding protein-related genes
Figure 5. The external validation of DDX20 and NCBP2. (A) The single-cell RNA-sequencing analysis of 432 testicular cell samples isolated from NOA patients displayed that DDX20 and NCBP2 were both positively associated with spermatogenesis. (B, C) DDX20 (B) and NCBP2 (C) were down-regulated in the seminal plasma samples of NOA patients from the local hospital. (D) The ROC curve exhibited the diagnosis ability of seminal plasma DDX20 and NCBP2 to NOA in the local cohort. (E) The protein expression levels of DDX20 were obviously down-regulated in the testicular samples from NOA patients in the local cohort, which were detected by the immunohistochemical staining. Abbreviation: ROC: receiver operating characteristic.