Research Paper Volume 15, Issue 8 pp 2999—3020

Figure 10. Rasgrf1 upregulation by the inhibition of miR-323-3p could improve the synapse structure deterioration after CCH. (A) The synapses were observed with uranyl acetate negative staining under transmission electron microscope and the synapses’ structure was observed; (Con (n = 3), 2VO (n = 3), 2VO+miRNA-323-3p antagomir (n = 3), 2VO+ miRNA-323-3p antagomir NC (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 siRNA (n = 3), 2VO+miRNA-323-3p antagomir + RasGRF1 scramble siRNA (n = 3)). (B and C) The cumulative and specific distribution of synaptic PSD length. (D) PSD length of synapses. (E) Synapse area and (F) the relative PSD intensity of synapses were measured and calculated. Scale bar = 200 nm. ^**p < 0.01. All of the experiments were repeated three times.