Research Paper Volume 15, Issue 18 pp 9341—9357

Figure 5. NUMB knockdown abolished the palmitic acid promoting effect in RPEC migration. (A) NUMB protein levels in ARPE-19 cells at 48 hours after transfection of negative control (NC) or NUMB-specific siRNA (siRNA1 and siRNA2) oligos. (B–E) ARPE-19 cells were separately transfected with NC, siRNA1, and siRNA2. After 48 h, cells were left untreated or treated with 200 μM palmitic acid for another 48 h in the subsequent assays. (B) miR-222 levels in the indicated cells after palmitic acid administration for 48 h. (C) Wound healing ability and (D) migration ability of the indicated ARPE-19 cells. (E) E-cadherin, vimentin, MMP2, and MMP3 protein levels in the indicated ARPE-19 cells. Representative images are shown, and the summarized data were from three independent results. n = 3 for each group; ^*P < 0.05, ^**P < 0.01. Abbreviation: ns: not significant, between the indicated groups.