Research Paper Volume 15, Issue 18 pp 9341—9357

Figure 2. Palmitic acid led to upregulated miR-222expression, which contributed to enhanced RPEC migration and EMT. (A) The transcript levels of the indicated miRNAs in control ARPE-19 cells and ARPE-19 cells after treatment with 200 μM PA for 48 h. (B, C) The wound healing ability of ARPE-19 cells transfected with control or the indicated miRNA mimics (B), or transfected with control or the indicated miRNA inhibitors (C), was measured by scratch assays. Representative images of cells before (0 h) and after (48 h) the scratch are shown, and the relative wound closure was summarized. Scale bar, 200 μm. (D) The migration of ARPE-19 cells transfected with control or miR-222 mimics was determined by transwell migration assays. Representative images are shown, and the invaded cell numbers per view field were summarized. Scale bar, 200 μm. (E) EMT-associated protein levels in indicated ARPE-19 cells. Representative bands images are shown, and the relative protein levels were quantitated. n = 3 for each group; ^*P < 0.05, ^**P < 0.01, between the indicated groups.