Research Paper Volume 15, Issue 7 pp 2582—2609

Figure 2. Basic information of AIF-1. (A) AIF-1 expression in 31 types of tissues. (B) AIF-1 expression in the cancer cell lines. (C) The level of AIF-1 expression between tumor and normal tissues in each type of cancer is based on the integrated data from TCGA and GTEx datasets. (D) The expression level of AIF-1 between KIRC and normal tissues. (E) The protein expression level of AIF-1 between tumor and normal tissues. (F and G) Determination and quantification of AIF-1 expression levels in KIRC tissues and paired normal tissues by western blotting assay. Tubulin was used as a loading control. (H) qRT-PCR analysis of AIF-1 mRNA expression in KIRC tumors and paired normal tissues. (I) AIF-1 expression levels in different pathologic grades, TNM stages, age phases, and body weights. (J) The immunofluorescence images of AIF-1 protein, nucleus, endoplasmic reticulum (ER), microtubules, and the incorporative images in U2-OS, NB-4, and REH cell lines. (^*P < 0.05, ^**P < 0.01, and ^***P < 0.001).