Research Paper Volume 15, Issue 14 pp 6721—6735

Figure 5. Macrophage scavenging alleviates the Th1-induced chronic colitis in mice. (A) Dynamic detection of mouse body weight (n=10). After CCL was used to scavenge macrophages, it resisted the effect of Th1 cells. According to mouse body weight detection, the reduction of body weight in DSS+Th1+CCL group was markedly alleviated relative to DSS+Th1 group, and the difference was significant. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group. (B) Mouse DAI score (n=10). The DAI scores of DSS+Th1+CCL group markedly decreased relative to DSS+Th1 group. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group. (C) FITC-D (n=10). In the detection of mouse intestinal mucosal permeability, the FITC-D level of DSS+Th1+CCL group markedly decreased relative to DSS+Th1 group, and the permeability decreased. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group. (D) Mouse intestinal length (n=10). The intestinal length of DSS+Th1+CCL group was extended compared with DSS+Th1 group. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group. (E, F) Detection of Th1 cell markers and inflammation cytokines (n=10). The levels of IL-2 and IFN-γ as well as inflammation cytokines TNF-α, IL-1β and IL-6 were changed, and the cytokine levels in DSS+Th1+CCL group were evidently reduced relative to DSS+Th1 group, and the difference was of statistical significance. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group. (G) Th2 cells-related factors (n=10). There was no significant difference in the IL-4, IL-10 and IL-13 levels. (H, I) Protein detection results (n=5). CCL improved the TJP levels, the levels of ZO1 and Occludin proteins in DSS+Th1+CCL group were markedly higher than those in DSS+Th1 group, while the level of p-STAT3 decreased. ^*P<0.05 compared with Control group, ^#P<0.05 compared with DSS group.