Research Paper Volume 15, Issue 7 pp 2525—2540

Figure 4. DDX5 alters circPHF14 expression. (A) Endogenous circPHF14 expression levels in multiple gastric cancer cells by RT-PCR. (B) We confirmed circPHF14 was formed by circularization of exons 14, 15, 16 in the PHF14 mRNA by Sanger sequencing, and also determined its genomic size and sequence. (C) RT-PCR was performed in AGS or 7901 cells transfected with DDX5, and then relative expression of circPHF14 was detected by qPCR. (D, E) The relative expression of circPHF14 and PHF14 mRNA in AGS or 7901 cells was detected by RT-PCR after RNase R treatment for 30 min (D) or actinomycin D treatment for 0 h, 8 h, 16 h, and 24 h (E). Data are mean ± SD of three independent experiments. * P < 0.05, ** P < 0.01, ns stands for no significance.